The investigation focused on the foam strike from the very beginning. Incidents of debris strikes from ice and foam causing damage during take-off were already well known, and had damaged orbiters, most noticeably during STS-45, STS-27, and STS-87.[29] Tile damage had also been traced to ablating insulating material from the cryogenic fuel tank in the past. The composition of the foam insulation had been changed in 1997 to exclude the use of freon, a chemical that is suspected to cause ozone depletion; while NASA was exempted from legislation phasing out CFCs, the agency chose to change the foam nonetheless. STS-107 used an older "lightweight tank" (a design that was succeeded by the "superlightweight tank", both being upgrades from the original space shuttle external tank) where the foam was sprayed on to the larger cylindrical surfaces using the newer freon-free foam. However, the bipod ramps were manufactured from BX-250 foam which was excluded from the EPA regulations and did use the original freon formula. The composition change did not contribute to the accident.[30] In any case, the original formulation had shown frequent foam losses, as discussed earlier in this article.