PhoKingGuy
Diamond Member
- Nov 15, 2007
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Pure H2O is not a good buffer (it will go acidic due to CO2 dissolution if not buffered by Tris or others), not a good stable medium (see above, not only will it hydrolyze bonds, but generally you want to add something like EDTA (to scavenge divalent cations and inhibit any enzymes that may have gotten in) and/or NaAz (to prevent the growth of any bacteria or yeast)), and I really wouldn't cause it a "volumizer" given that pretty much all molecular biology experiments are fluid-phase anyway. It's more of a diluent to achieve the proper concentration.
For the thread:
Man really landed on the moon
Lee Harvey Oswald acted alone
9/11 was planned by Osama Bin Laden
Obama was born in Hawaii
Vaccines are safe and do not cause autism
Its conductivity or lack thereof has nothing to do with its utility for molecular biology.
It was like 2AM and I was drunk off my ass, I don't know what the fuck I was trying to say with that, haha