I don't defrag any of my "main" volumes, which are all FAT32. I have a couple of NTFS volumes, to allow for > 4GB filesizes, those I simply in-place re-format if I ever feel that the MFT for those volumes has become fragmented for some odd reason. I don't see any real reason to waste time with defragmentation. I do use partitions though, and tend to keep various similar types of files together, thus reducing the occurance of fragmentation as well, as much as can be done.