Many viruses defeat installed anti-malware software. If one of them has infected your machine, it probabably won't allow you to install any other AV software, but you can try.
Some viruses will try to trick you by allowing you to do what looks like an update of their definitions, but if you look at thejlast date, it may not actually have advanced the date or ID number of the current definitions.
What AV are you using? .You can try to re-install it or download another one, but if you're infected, it may already be too late for that.
My personal favorite is the free version of Avast. Others may prefer another and/or have other suggestions.
The really sure way to get rid of infections is to re-install Windows from the ground up. If you do that, consider installing it on another drive. Once you've got it installed and updated and installed a new round of AV and anti-spyware, you can try to rescue your old files by booting up, AFTER it's already booted and protected, connecting your current drive as a slave, and sweeping it for malware.
If you REALLY want to be safe, consider buying another drive the same size as your current drive (or larger), and regularly clone your drive immediately after each successful anti-malware sweep and before installing any new software.
"Cloning" a drive creates a fully running, bootable clone of your hard drive. That is, it makes an exact, running duplicate of your hard drive. If your main drive fails, the cloned image can directly replace the old drive, or it can be used to reload everything, including your operating files, on a new drive without re-installing the system or your programs. If your hard drive fails or becomes irreparably infected, or a new software installation goes south, it WILL save your butt. There is good, free cloning software available.
Good luck.