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Microscopic Photography

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Originally posted by: Garth
Originally posted by: Mark R
Sweet scopes. Sweet pics.

/me wonders how much a nice binocular transmission microscope with 10-100x objectives (inc oil immersion) and Canon EOS adapter.
Click to expand...
A Zeiss Axiostar with trinocular head, EOS mount and A-Plan objectives will probably start around $4K, give or take. Fluorescence equipment (as pictured there) would take another $5K or so.

The microscope/camera setup on which I took the pic of fluorescent BPAE cells lists for about $34K. That scope is equipped to take pictures like THIS (i.e. with 3 colors) and I intend to take some this weekend.
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What are the dyes there? I'd guess flourascein (sp), rhodamine and...???
 
The slide I have is a prepared slide manufactured by Molecular Probes (it's #1 on that page).

The filter cubes that I'm using are tuned for Fluorescein (FITC), DAPI, and Rhodamine, respectively.

I'm not sure which dyes are used in that one pic I just posted. I just grabbed it off the web somewhere as an example of multi-channel fluorescence.
 
Originally posted by: Garth
The slide I have is a prepared slide manufactured by Molecular Probes (it's #1 on that page).

The filter cubes that I'm using are tuned for Fluorescein (FITC), DAPI, and Rhodamine, respectively.

I'm not sure which dyes are used in that one pic I just posted. I just grabbed it off the web somewhere as an example of multi-channel fluorescence.
Click to expand...

ah, DAPI would be the blue, it's specific for the nucleus.
 
You should take a pic of dug777's penis. You might be able to actually see it using your microscope set up.
 
You guys need to dump the Diagnostic Instruments stuff and move up to some real cameras!
😉

Other than that, nice pics.
 
Actually, the new pics were taken with a Q-Imaging monochromatic camera, and then pseudo-colored. The D.I. cameras are pretty good as long as you select the right one for the right application. Mono is better for fluorescence than a chip using Bayer interpolation, for example, but I haven't had a reason to requisition a mono D.I. camera.
 
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