Like Budman said, just do a search for MSDOS.SYS, and open it with notepad.  Make sure to change the properties so it's not read only.  Add Logo=0.  I didn't know you could put it at the end, I usually put it after WinVer.  Anyway, you can also add BootDelay=0 to take off a couple of seconds off your boot time.  Change the properties back to Read Only, and your set.
I never much liked Tweak UI because of the stock settings.  You actually have to go in and change a bunch of stuff just to get back to normal operations.