It doesn't make a difference as long as all the sticks are matched. Dual channel mobo's only have 2 memory controllers so splitting them up doesn't have any benefit. Beware though, some companies (like Corsair) only guarantee their modules to run at the rated speeds in singles or pairs. I currently run 4 x 256 with no problems, but if I was to start over again I would do 2 x 512 simply because all of my slots are full now. 2 x 512 leaves you room to upgrade if you should need to in the future.