Want to See Something Microscopic in 3D?

[Circlenaut]

Over the next three week's I'll have access to my school's very expensive (and recently bought) confocal microscope. This is part of a lab module and the Professor is encouraging us to bring in samples. I have some ideas about scoping up some algae or finding some diatoms. Any one of you guys like to request to see something? Samples will look best if they have some kind of auto-fluorescing property (I.E. Green Fluorescent Protein or Chlorophyl). A 3D image is rendered by taking multiple 2D slices of a sample.

 

[amdhunter]

edited stupid personal attack.

 

[Circlenaut]

how about your little pippy? Or is the microscope not strong enough.

wtf?

 

[Numenorean]

wtf?

So not strong enough then.

 

[highland145]

shens w/o pics.

 

[RavenSEAL]

Hair 3D!

 

[darkswordsman17]

wtf?

Ignore him and Numenorean, they've had botched lobotomies.

Maybe something that has some crystalline structure. Some cloth, or say a dollar bill might be interesting to see the fibers.

 

[DisgruntledVirus]

Tits or gtfo

 

[Taejin]

How about your little pippy? Or is the microscope not strong enough.

are you an actual idiot or is all of this just a big ruse?

 

[Circlenaut]

Wow, I thought maybe someone might think its cool to see something microscopic in 3D (maybe a cpu die or something). I guess you fuckers will just choose to be childish. Whatever, if someone actually cares, PM me.

 

[PottedMeat]

Wow, I thought maybe someone might think its cool to see something microscopic in 3D (maybe a cpu die or something). I guess you fuckers will just choose to be childish. Whatever, if someone actually cares, PM me.

Order some ADXL samples from analog devices. Use a soldering iron to take off the metal top. Don't jar the thing, it might break. Take a picture of that - most of it should be a MEMS capacitor. You may be able to see one of the leaves move.

 

[IronWing]

Inclusions in quartz, beryl

 

[Crusty]

Wow, I thought maybe someone might think its cool to see something microscopic in 3D (maybe a cpu die or something). I guess you fuckers will just choose to be childish. Whatever, if someone actually cares, PM me.

LOL, don't get so butthurt because of some idiot trolls. The trolling that happens on ATOT is incredibly lame compared to the rest of the Internet....

 

[polarmystery]

take pictures of a CPU die. That'd be cool to see in 3d due to the vertical layer of copper connections in all of the transistors.

 

[Azraele]

It's a shame that this thread degraded so quickly. It had real potential to produce some really cool things.

 

[dud]

OP, what magification range are you going to be using (10X, 100X, 1000X)?

 

[rudder]

Wow, I thought maybe someone might think its cool to see something microscopic in 3D (maybe a cpu die or something). I guess you fuckers will just choose to be childish. Whatever, if someone actually cares, PM me.

This is ATOT... you have this forum confused with the highly technical section. These responses are par for the course.

 

[skyking]

Put up the cool pics, that will shut up the asshats.

 

[zinfamous]

Wow, I thought maybe someone might think its cool to see something microscopic in 3D (maybe a cpu die or something). I guess you fuckers will just choose to be childish. Whatever, if someone actually cares, PM me.

You came to the wrong place!

actually, had you gone ahead and posted the pic in the OP with something like--"Look at this kick-ass confocal image I took--guess what it is!" you would have people creaming all over this thread.

Instead, it's just opened up for some good-natured trolling. Such is the way of AT. :\


that being said, I'd like to see a Drosophila's head. Or, even better, a cross section of 1st instar larvae (Dros.) testis. This is pertinent to my current work.

 

[SSSnail]

How about an ant?

 

[silverpig]

You came to the wrong place!

actually, had you gone ahead and posted the pic in the OP with something like--"Look at this kick-ass confocal image I took--guess what it is!" you would have people creaming all over this thread.

Instead, it's just opened up for some good-natured trolling. Such is the way of AT. :\


that being said, I'd like to see a Drosophila's head. Or, even better, a cross section of 1st instar larvae (Dros.) testis. This is pertinent to my current work.

testis? you're looking at fly cock 'n' balls?

It's too bad you didn't post this about 2 years ago. I'd have had some super sweet samples to send you.

 

[zinfamous]

testis? you're looking at fly cock 'n' balls?

It's too bad you didn't post this about 2 years ago. I'd have had some super sweet samples to send you.

we're trying to section--so, 3-4 cuts--individual testis in fly larvae, and adults (which can be done manually, though it's painful). we want to do gene expression profiles of y chromosome in various developmental stages (each section of testis contains spermatazoa at different developmental stages).

the plan is to get an uber expensive laser microdissection scope to help us with this. Currently, i'm curious about size, magnification needs, general issues of "What do we need to do this?"

 

[Circlenaut]

OP, what magification range are you going to be using (10X, 100X, 1000X)?

The objectives goes up to 100x with oil immersion so yeah 1000x is max.

 

[Circlenaut]

You came to the wrong place!

actually, had you gone ahead and posted the pic in the OP with something like--"Look at this kick-ass confocal image I took--guess what it is!" you would have people creaming all over this thread.

Instead, it's just opened up for some good-natured trolling. Such is the way of AT. :\


that being said, I'd like to see a Drosophila's head. Or, even better, a cross section of 1st instar larvae (Dros.) testis. This is pertinent to my current work.

Hmm, I know one of the Professors in the Dept. works with Drosophilae. I'll see about getting a head and mounting it. I'll definitely be cool to see the eyes.

 

[zinfamous]

Hmm, I know one of the Professors in the Dept. works with Drosophilae. I'll see about getting a head and mounting it. I'll definitely be cool to see the eyes.

:thumbsup:

they have really cool heads.