USB does pauses when you try to access your HDD VERY HEAVILY, ie load 3 or more programs at the same time. I have tried it on a few machines, a few P3, a few Celerons. P3 'pauses' muchless while the celeon 366/400/466 does tend to pause more often. USB in itself takes an IRQ anyway, so if you are ONLY using a mouse on the USB then you are not really 'saving' any IRQ at all. USB having a 'default' higher rate than PS2 is not really an 'advantage'. PS2/rate (the program to adjust PS2 refreshes) is FREE for download and it is SO easy to use, and only needed to be used once, and I can get 200Hz refresh, way more than 125hz of usb.
A mouse doesnt slow your CPU down, but USB does. USB relies on your CPU for all data transfer. If you even tried a USB webcam vs a PCI webcam, you will see the CPU usage difference. On my 868E, running a USB webcam takes nearly 50% of my CPU during capture, running a PCI webcam capturing at the same quality, less than 20%. One might say Webcam has a much higher data transfer, but basically it just 'magnify' the CPU usage.