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Semi-dificult question....help me.

How do you outline an experiment using radioactive carbon to prove that carbon in glucose comes from carbon dioxide?

Can someone help me?
 
Originally posted by: ckk81
Originally posted by: minendo
Mark the CO2 with a C14 molecule.

could you explain it w/ more details.

Use CO2 that is made with the C14 isotope insted of the common C12. In the end, your final glucose molecule will be heavier if it was infact made with the C14 isotope of CO2.
 
Where are you taking the glucose from to test it after the experiment is over? I'm guessing a plant. So, you would grow the plant in an atmosphere enriched with C14 based carbon dioxide. There may be cheaper/easier ways to radiolable the carbon though. I'm not an expert.

R
 
Originally posted by: minendo
Originally posted by: ckk81
Originally posted by: minendo
Mark the CO2 with a C14 molecule.

could you explain it w/ more details.

Use CO2 that is made with the C14 isotope insted of the common C12. In the end, your final glucose molecule will be heavier if it was infact made with the C14 isotope of CO2.


How would you know if the final glucose molecule will be heavier just because it used C14 instead of C12? please explain.

 
Originally posted by: ckk81
Originally posted by: minendo
Originally posted by: ckk81
Originally posted by: minendo
Mark the CO2 with a C14 molecule.

could you explain it w/ more details.

Use CO2 that is made with the C14 isotope insted of the common C12. In the end, your final glucose molecule will be heavier if it was infact made with the C14 isotope of CO2.


How would you know if the final glucose molecule will be heavier just because it used C14 instead of C12? please explain.

Because C14 is heavier than C12. Standard glucose is composed of C12. The atomic mass is roughly (6x12)+(12x1)+(6x16). If the mass differs from this, you have C14 present.

I may be off a little on the exact methods, but look in just about any chemistry/biology textbook and this experiment should be covered.
 
Originally posted by: minendo
Originally posted by: ckk81
Originally posted by: minendo
Originally posted by: ckk81
Originally posted by: minendo
Mark the CO2 with a C14 molecule.

could you explain it w/ more details.

Use CO2 that is made with the C14 isotope insted of the common C12. In the end, your final glucose molecule will be heavier if it was infact made with the C14 isotope of CO2.


How would you know if the final glucose molecule will be heavier just because it used C14 instead of C12? please explain.

Because C14 is heavier than C12. Standard glucose is composed of C12. The atomic mass is roughly (6x12)+(12x1)+(6x16). If the mass differs from this, you have C14 present.

I may be off a little on the exact methods, but look in just about any chemistry/biology textbook and this experiment should be covered.

i'm actually impressed
 
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