You COULD Crossfire on the P35 chipset (16x4 PCI-E 1.1) but it wouldn't be advisable IMO. The 2900's and 38xx series suffer at max a 20% performance hit by running in a 4x slot (generally 10-15%) and if you would want the new 4xxx cards that would probably be even more pronounced.
Here is a recent comparison between 16x16 PCI-E 2.0 Crossfire and 8x8 PCI-E 2.0 Crossfire (note that 8x PCI-E 2.0 is the same bandwidth as 16x PCI-E 1.1). As you can see it's a pretty big impact between 16x16 and 8x8, and if you want to run a card in 4x PCI-E 1.1 then thats 1/4 of the performance of the P45 indicated in the review.
Bottom line it would probably be better to stick to single GPU in a P35 board and if you want Crossfire it would be better to get a new mobo.