There are 8 PCIe2 lanes on P67 chip, and everything that goes through P67 have to share those 8 lanes.
For graphics the 2 PCIe2 x16 sockets connect direct to the cpu.
The 3rd PCIe x16 socket runs at x4, and is connected to P67. If you add a card to that socket, and choose to run that card at x4 (it's x1 by default without the PLX chip), then SATA and USB3 will be limited, or possibly starved of bandwidth.
If you're not connecting a card to the 3rd PCIe x16 socket, then you don't need the PLX chip.