first, very very few motherboards supporting the most current cpus also support pc133 sd-ram
but if you compare, let's say, the difference between pc133 and pc2100 in an old athlon cpu (ie. athlon 1.33) with one using pc133 and the other one using pc2100, the real-world difference is about 15-20% as i recall
and considering the improvement of ddr chipsets over the past 2 years, i'd yes the real-world difference is significant, or at least very noticeable