I don't bother with unsubscribe addresses. I just go to
www.spamcop.net and copy in the mail (headers and all). It figures out how to complain to and sends off the complains. UUNet & PSInet are very good about shutting down spammers' dialup accounts. Geocities is good about killing websites.
I made the mistake of signing up for TreeLoot one time. They sold my address to some company called VirtuoMondo who recently started spamming me no less than 4 times a day. I'm thinking about killing my email account for a few weeks so it bounces a bunch of spams back then reopening it so I can use it again (it's on my domain and I like the username so I'd rather not trash it completely).
Here's a hint. In the future when you sign up for crap, use an account from
www.mailexpire.com to create a forwarder that will expire in a set amount of time. I'm sure you can find some uses for it......