Sorry didn't mean it in a bad way but most malware can't be picked up in safe mode especially rootkits. Then without knowing the name of the malware booting into safe mode trying to clean your pc with a file virus like Virut will destroy your data beyond repair. There's no disinfecting from there nor any other way as to wipe all your data then. Not even online experts can save you. That's why you never delete a file flagged as suspicious or go into safe mode. You upload it to a webscanner to get its name and its procedures. What I normally do I let it loose in a virtual machine tracing it then use a script to with Combofix to hunt it down.
For rootkits gmner. No single application, virus or anything has stood against gmner. It can bring down any rootkits. the ram thing always when your done cleaning switch of the pc completely for a few minutes to clear the ram completely. You can pop the sticks out aswell if you don't feel like waiting.
If you like to learn more about removing malware manually here are some good sources
http://www.backgroundtask.eu/Systeemtaken/Scan.php
Very good tool to start with
http://www.niksoft.at/download/startdreck.htm