I believe the correct answer is yes, if they are on the same ribbon. I believe that there has been some problem in the past using differing EIDE/33/66 drives on the same ribbon. I ran into it with EIDE and /33. A cheap solution might be a simple Promise/66 controller card for the /66. Pretty cheap now. Actually, from what I've read, you won't see /66 xfer speed anyway. Supposedly, they normally can't even max out the /33, unless they are RAIDed.
IME, where you'll notice the difference in the drives is in the rpm, probably 7200 vs. 5400.
--Randy