"The only stupid question, is the question that was not asked"
OK, that was the lecture. 😉
I imagine FAT32 being a tad bit slower than FAT16, as it has a bit more overhead to carry along, but i think its negliable. However, keep in mind that the maximum partition size with FAT16 is 2GB, and with the 2GB it uses 32KB clusters. I dont think 2GB partitions is very useful anymore in todays world where diskspace is so cheap and programs grow so huge.