I don't know anything about the EZ Plug, but if it's the 4-pin connector to give extra power to the CPU, I would recommend using it.
Since you are using the 24-pin connector, this isn't a question about the 4 extra pins you would see on the mobo if you were using a 20-pin connector from the PSU. To be clear, you don't need THOSE 4 pins (unless you are using PCI-E and your card doesn't have it's own power connector).
The ASUS A8N-SLI Premium has 3 different power plugs
1 x 24 pin -- Need (but the 20 pin works)
1 x 4 (square) pin CPU 12V ATX -- Need
1 x 4 pin EZ Plug (which most people call a Molex) -- Recommended if running 2 video cards, other wise optional
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