Disable windows updates for any mission critical system. Maintain offline backups for any mission critical data.
Something is odd, changing drive letter should just mean using the different drive letter for access.
Go into Disk Management and see if the drive is listed, and what it states. I wonder if AOMEI did something more to the drive than it should have, and I wonder why you would have used that when windows itself is capable of merely changing the assigned driver letter.
Beyond that, I would connect the drive to another system to see if it can be accessed. Make SURE the other system has "Autorun" disabled, because I am wondering if this virus checker is really ransomware and has encrypted your files or something... which is another reason why an offline backup is so important, once you are sure your system is free from malware so it doesn't just mess with your backup too when you connect it.
So...
1) This AMP simulator, did you install it? You might upload to virustotal to see if it's got malware in it.
2) Indentify whatever this unexpected virus scanner is, from the list of running processes in task manager. Maybe just Windows Defender/something, or maybe the malware itself.
3) Run a virus and malware scanner on the system.
4) No reason to reformat it, that does not solve anything. If there is just some logical problem, "maybe" AOMEI can fix it but I would hesitate to try that until other potential avenues are pursued (depending on what windows' Disk Management shows for the drive).
5) Possibly I am lost on a tangent and the first thing to do is check and replug the data cable to the drive and motherboard, or try a different cable, with system powered off at the time of course (power switch on PSU flipped off, or surge strip power switch, or unplug from wall, but then make sure to ground yourself before working in the PC, especially if there's a lot of ESD, static electricity in the room).