Yes and no. Older PC133 with lower density chips (128Mb x 8 or 16) will work on most PC100 based systems, but the newer high density chips (256Mbx4 or 8, 256MB or 512MB DIMMs) may not work, also depends on chipset support. If you're still using an old skool 440BX based board, I'm don't think you can even use 256MB standard unbuffered modules; you'd have to use registered?