The memory hole only affects certain *VERY* old, very rare cards.
Back when a top-end workstation would have had 16MB RAM, they inserted this memory hole to allow some high end ISA cards to use system memory instead of incurring the cost of adding 1MB on the card (around $100 at the time).
So unless you have one of these weird-a$$ cards, you shouldn't worry about the hole. Its best to leave it off.
Eric