Ok, first of all, a 3000+ is not an FX-53... they are different processors. The FX line is a waste unless you're doing extreme overclocking and need unlocked multipliers or you have money to burn and want bragging rights.
If you're building a whole new system, then A64 is a great value. A good A64 motherboard would run you around $100 and a 3000+ A64 is around $150.
Tom's hardware has a bunch of CPU benchmarks for a variety of applications so you can see what kind of performance impact a faster processor would make.
A64 really excels at games.
Windows 64-bit isn't out yet afaik. A64 will run everything a 32-bit processor can run. It runs Windows XP 32-bit just fine.
Not much is out yet that's meant for 64-bit, but once there is, the A64 will really shine.
What is your current setup? You actually might be better served by a videocard upgrade instead of CPU if you're doing this for a gaming upgrade.